Coding

Part:BBa_K5094002:Design

Designed by: HuiChing Kuo   Group: iGEM24_KCIS-Xiugang-Taipei   (2024-09-20)


IsPETase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 220
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

several repeat regions are in this gene so it's difficulty to make specific mutations via site-directed mutagenesis


Source

he wild type of I. Sakaiensis bacteria( IsPETase) gene

References